dsm 43185  (DSMZ)

Journal: Current Research in Food Science

Article Title: Genistein alleviates colitis by suppressing inflammation and modulating colonic Marvinbryantia formatexigens abundance and metabolites

doi: 10.1016/j.crfs.2025.101016

Figure Lengend Snippet: Genistein regulates gut bacteria composition and promotes SCFA production. (A) Genistein relieves colitis by reducing the alpha diversity index of gut bacteria. (B) Effects of genistein on gut bacteria at the phylum level. (C) Effects of genistein on gut bacteria at the genus level. (D) Genistein was able to increase the relative abundance of Marvinbryantia formatexigens in colitis. (E) Principal coordinates analysis (PCoA) showing significant clustering across treatment groups. (F) Genistein increases the concentrations of acetate, butyrate and SCFA in colitis stool. The number of mice in each treatment group was n = 6.

Article Snippet: Marvinbryantia formatexigens bacteria were purchased from German Collection of Microorganisms and Cell Cultures (DSMZ), and the culture method was cultured according to the recommended medium.

Techniques: Bacteria

Journal: Current Research in Food Science

Article Title: Genistein alleviates colitis by suppressing inflammation and modulating colonic Marvinbryantia formatexigens abundance and metabolites

doi: 10.1016/j.crfs.2025.101016

Figure Lengend Snippet: Marvinbryantia formatexigens attenuated DSS-induced colonic inflammation. (A) Schematic diagram of the time axis of the bacterium gavage test. (B) Gavage of Marvinbryantia formatexigens in mice alleviated colitis-induced body weight loss. (C) Marvinbryantia formatexigens is able to alleviate colonic atrophy induced by DSS-induced colitis. (D) Marvinbryantia formatexigens was able to alleviate the pathological score elevation caused by DSS-induced colitis. (E) Marvinbryantia formatexigens was able to alleviate DSS-induced colitis-induced increase in pathological score and decrease in the number of positive cells stained with neutral mucus ( × 100 magnification; scale bar: 500 μm). (F) Marvinbryantia formatexigens can inhibit DSS-induced colonic inflammation by inhibiting NF-κB/IκB phosphorylation-mediated Cox-2 signaling. (G) Gavage of Marvinbryantia formatexigens in mice can inhibit the NLRP3 signaling pathway and alleviate the inflammatory response. (H) Marvinbryantia formatexigens can suppress TLR4/COX-2/PGE2 gene expression and alleviate DSS-induced colonic inflammation. The number of mice in each treatment group was n = 8.

Article Snippet: Marvinbryantia formatexigens bacteria were purchased from German Collection of Microorganisms and Cell Cultures (DSMZ), and the culture method was cultured according to the recommended medium.

Techniques: Staining, Phospho-proteomics, Gene Expression

Journal: Current Research in Food Science

Article Title: Genistein alleviates colitis by suppressing inflammation and modulating colonic Marvinbryantia formatexigens abundance and metabolites

doi: 10.1016/j.crfs.2025.101016

Figure Lengend Snippet: Marvinbryantia formatexigens improved mucosal barrier and epithelial dysfunction caused by colitis. (A) Marvinbryantia formatexigens alleviate colitis-induced decrease in Muc2 protein and decrease in goblet cell marker UEA-1 ( × 400 magnification; scale bar: 50 μm). (B) Marvinbryantia formatexigens attenuates the expression of the tight junction genes Claudin1 and Occludin caused by colitis. (C) Marvinbryantia formatexigens inhibits colitis-induced increases in Edu and PCNA-positive cells, markers of intestinal proliferative cells ( × 400 magnification; scale bar: 50 μm). (D) Inhibition of Marvinbryantia formatexigens inhibits the elevation of Wnt2b/β-catenin protein expression in colitis. (E) Marvinbryantia formatexigens inhibits colitis-induced increase in Wnt gene expression. (F) Marvinbryantia formatexigens inhibits autophagy caused by colitis-induced decreased AKT/mTOR/P70S6K expression. (G) Marvinbryantia formatexigens increases Stat3-mediated apoptosis in colitis. (H) Marvinbryantia formatexigens inhibits the elevated expression of Lgr5 and ASCL2 in colitis. (I) Marvinbryantia formatexigens inhibited the elevated expression of the slow-proliferating genes Tert, Bmi, Lrig1 and Sox4 caused by colitis. (J) Marvinbryantia formatexigens increased the concentration of SCFA in feces. The number of mice in each treatment group was n = 8.

Article Snippet: Marvinbryantia formatexigens bacteria were purchased from German Collection of Microorganisms and Cell Cultures (DSMZ), and the culture method was cultured according to the recommended medium.

Techniques: Marker, Expressing, Inhibition, Gene Expression, Concentration Assay

Journal: The ISME Journal

Article Title: Metaproteomics-based stable isotope fingerprinting links intestinal bacteria to their carbon source and captures diet-induced substrate switching

doi: 10.1093/ismejo/wraf127

Figure Lengend Snippet: Changes in isotopic signatures of gut microorganisms in response to changes in the isotopic signature or source of dietary protein. (A) Overview of Experiment 1 as described in Materials & Methods . (B, D, F, H, J) Protein-SIF δ 13 C values for mouse, A. muciniphila , B. thetaiotaomicron , M. formatexigens and B. uniformis . Displayed δ 13 C values for dietary protein sources, corn oil, corn starch, sucrose, and corn fiber were measured by isotope ratio mass spectrometry (IRMS). Significance is denoted by * and determined by T-tests corrected by BH (q < 0.05; n = 5 unless otherwise stated). (C, E, G, I) Relative proteinaceous biomass of A. muciniphila , B. thetaiotaomicron , M. formatexigens and B. uniformis determined according to the method described by . Letters that do not overlap denote significantly different groups as determined by Tukey HSD ( P < .05).

Article Snippet: Marvinbryantia formatexigens , 14 469, Type strain I-52 , DSMZ , Bacillota.

Techniques: Starch, Mass Spectrometry

Journal: The ISME Journal

Article Title: Metaproteomics-based stable isotope fingerprinting links intestinal bacteria to their carbon source and captures diet-induced substrate switching

doi: 10.1093/ismejo/wraf127

Figure Lengend Snippet: Changes in isotopic signatures of gut microorganisms in response to changes in the isotopic signature or source of dietary fiber or fat. A. Overview of Experiment 2 as described in Materials and methods . (B, D, F, H, J, L, N) Protein-SIF δ 13 C values for mouse, A. muciniphila , B. thetaiotaomicron , M. formatexigens , B. uniformis , B. caccae , and E. coli , respectively. Displayed δ 13 C values for dietary fiber and fat sources, corn starch, sucrose, and egg white protein were measured by isotope ratio mass spectrometry (IRMS). Significance is denoted by * and determined by T-tests corrected by BH (q < 0.05; n = 6 unless otherwise stated). (C, E, G, I, K, M) Relative proteinaceous biomass of A. muciniphila, B. thetaiotaomicron, M. formatexigens and B. uniformis, B. caccae, and E. coli . Letters that do not overlap denote significantly different groups as determined by Tukey HSD ( P < .05).

Article Snippet: Marvinbryantia formatexigens , 14 469, Type strain I-52 , DSMZ , Bacillota.

Techniques: Starch, Mass Spectrometry

Journal: The ISME Journal

Article Title: Metaproteomics-based stable isotope fingerprinting links intestinal bacteria to their carbon source and captures diet-induced substrate switching

doi: 10.1093/ismejo/wraf127

Figure Lengend Snippet: Hierarchical clustering of M. formatexigens proteins that significantly differed between casein, egg, white, and soy protein. (A) Table listing 17 proteins that were more abundant in the soy cluster. (B) Hierarchical clustering of the z-score values of the 60 proteins that changed significantly in abundance between the three dietary protein sources (ANOVA, q < 0.05, n = 5). (C) Abundant proteins that potentially explain the increase in the δ 13 C value in the soy diet.. Glutamine synthetase is highlighted in red; proteins from a Sugar ABC transporter gene neighborhood are highlighted in blue. Bars represent average %orgNSAF abundance and error bars represent the standard deviation.

Article Snippet: Marvinbryantia formatexigens , 14 469, Type strain I-52 , DSMZ , Bacillota.

Techniques: Standard Deviation